The degradation of 125I-insulin and 125I-b-amyloid was measured by the trichloroacetic acid (TCA) solubility approach. GSSG, GSH, and GSNO, and oxidized GSNO (GSNO(ox)) had been solubilized in the assay diluent (a hundred mM Tris-Cl)

Insulin-degrading enzyme (IDE, EC 3.four.24.fifty six) hydrolyzes a quantity of little (,12,000 MW) peptides, notably insulin and Alzheimer's amyloid b peptide. As these kinds of, it has been implicated in…