To work out phagosome acidification premiums, the portion of FITC/InlA -beads that were inside of acidified phagosomes at a variety of time details after original bead binding for MDCK and Caco-two cells was decided (Figure 3A and 3B ?black line)
Cells were being then rinsed with 4uC media and incubated with unlabeled InlA-beads utilizing the identical experimental protocol explained formerly for the FITC/InlA-bead experiments. Soon after a ninety five and…