Immediately after incubation for 45 min at 37uC the samples were being centrifuged at 14000 g for 40 min and analyzed using Orbitrap MS
The samples had been subsequently reduced and alkylated with one,4-dithiothreitol and iodoacetamide and digested overnight making use of trypsin (Promega, Madison, WI). The digestion was terminated by including trifluoracetic acid…