Na Morais, Paula Lorenzo and Maurizio Cocucci Received: 24 August 2021 Accepted: 14 October 2021 Published: 21 OctoberAbstract: Leucaena leucocephala (Fabaceae) is native to Central America and has invaded a lot of climatic regions of the tropics. In South Africa, the species is categorized as an emerging or incipient weed made use of as fodder, timber, firewood and in erosion handle on degraded habitats. The species is popular along the eastern subtropical regions of KwaZulu-Natal (KZN) Province, where it invades grasslands, savannas and edges of forests. Soils of those ecosystems are characterized as nutrient deficient and acidic. Working with a pot trial, we determined the effects on the nutrient addition remedies on microbial symbiosis, N nutrition and biomass accumulation of L. leucocephala beneath greenhouse conditions. Soon after 180 days of development, plants had been harvested, and their utilization of N derived in the atmosphere and from the soil was quantified by means of determination of 15 N values. L. leucocephala maintained growth and N nutrition by relying on each atmospheric- and soil-derived N LLY-283 Data Sheet across all soil therapies. The NDFA was substantially higher in higher P (N1 + P, N2 + P and N3 + P) soils. L. leucocephala was in a position to nodulate with intermediate and fast-growing strains in the Mesorhizobium and Rhizobium genus in N2 + P grown plants. This shows that L. leucocephala possesses traits which are productive in acquiring nutrients, particularly in nutrient restricted circumstances, by establishing plant symbiosis with various bacteria and relying on extracting N from the soil and from the atmosphere by means of the symbiosis. Search phrases: emerging invasive; KZN soils; Leucaena leucocephala; N fixation; N and P deficiencies1. Introduction L. leucocephala (Lam.) de Wit (Fabaceae) is native to Central America and has been introduced in a lot of geographic and climatic regions [1]. The species has been listed in the best one hundred worst invaders from the globe following deliberate introductions for agroforestry [1]. In South Africa, L. leucocephala is categorized as an emerging or incipient weed mainly occurring in the eastern subtropical components of the country [2] made up of grassland, forest and savanna. However, most invasions have already been noted inside the savanna biome [3]. Savanna and grassland ecosystems deliver several ecosystem solutions [4] and contribute for the rural and agricultural economy of South Africa in many ways [5], and the rural socioeconomy [6]. Hence, sustainable management of plant biodiversity is an integral a part of these ecosystems. South African savanna and grassland ecosystems are reported to become nutrient limited, in particular with regards to nitrogen (N) and phosphorus (P) [7,8]. Also, the soils are also acidic [9]. Legume plants need N and P for many processes such as nodule growth and function [10]. Regardless of the nutrient limitations inside the savanna and grassland ecosystems, the legume taxa continue to thrive and are most represented among the invasive species in nutrient poor ecosystems [11]. N-fixing bacteria and arbuscular mycorrhizal (AM) fungi symbiosis has been recognized because the driver ofPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This short Tetracosactide acetate article is an open access write-up distributed below the terms and conditions with the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/lice.