Recombinant Human IGF-BP1
IGF-BPs controls the distribution, function and activity of IGFs in various cell tissues and body fluids. Currently there are seven named IGF-BPs that form high affinity complexes with both IGF-I and IGF-II. IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, deciduas, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds to both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, where as nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a 25.4 kDa protein consisting of 235 amino acid residues (Isoform A). BYL-722
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Source: |
E. Coli
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Purity:
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Above 98% as determined by SDS-PAGE Analysis.
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Cross Reactivity: |
NULL
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Biological Activity
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The ED50 was determined by its ability to inhibit IGF-I induced proliferation of MCF-7 is ≤ 0.5 μg/ml in the presence of 6 ng/ml of human IGF-I.
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AA Sequence
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MAPWQCAPC SAEKLALCP PVSASCSEV TRSAGCGCC PMCALPLGA ACGVATARC ARGLSCRAL PGEQQPLHA LTRGQGACV QESDASAPH AAEAGSPES PESTEITEE ELLDNFHLM APSEEDHSI LWDAISTYD GSKALHVTN IKKWKEPCR IELYRVVES LAKAQETSG EEISKFYLP NCNKNGFYH SRQCETSMD GEAGLCWCV YPWNGKRIP GSPEIRGDP NCQIYFNVQ N
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| Research Interest: | , , Proliferation and Cell Cycle, |
| Protein Cross Reactivity: | NULL |