Anti-Human CD3 BG Violet 450
The SK7 monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex contains γ, δ, and ε chains, and it is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction. The SK7 antibody is reported to have a mitogenic effect on the majority of peripheral blood T cells in the presence of functional monocytes.
BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm. Staurosporine
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Clone
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SK7
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Format: |
BG Violet 450
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Applications
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FC
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Reactivity
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Human
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Isotype:
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Mouse IgG1, kappa
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| Cell Type: | , , , Th17 cells |
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Van Dongen, J. J., Krissansen, G. W., Wolvers-Tettero, I. L., Comans-Bitter, W. M., Adriaansen, H. J., Hooijkaas, H., … & Terhorst, C. (1988). Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood, 71(3), 603-612. Haynes, B. F. (1986). Summary of T cell studies performed during the second international workshop and conference on human leukocyte differentiation antigens. In Leukocyte typing II (pp. 3-30). Springer New York. Kap, Y. S., van Meurs, M., van Driel, N., Koopman, G., Melief, M. J., Brok, H. P., … & At Hart, B. (2009). A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. Journal of Histochemistry & Cytochemistry, 57(12), 1159-1167. |