Anti-Human TNF-Alpha APC
The MAb11 monoclonal antibody specifically reacts with human tumor necrosis factor alpha (TNF-alpha), a 157 amino acid non-glycosylated protein. TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. The MAB11 antibody was generated from recombinant human TNF and is reported to be a neutralizing antibody. ALW-II-41-28
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Clone
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MAb11
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Format: |
APC
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Applications
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FC
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Reactivity
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Human
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Isotype:
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Mouse IgG1, kappa
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| Research Interest: | , Cytokines & Growth Factors, |
| Cell Type: | Th1 cells, , , |
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Danis, V. A., Franic, G. M., Rathjen, D. A., & Brooks, P. M. (1991). Effects of granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), IL‐2, interferon‐gamma (IFN‐gamma;), tumour necrosis factor‐alpha (TNF‐alpha;) and IL‐6 on the production of immunoreactive IL‐1 and TNF‐alpha; by human monocytes. Clinical & Experimental Immunology, 85(1), 143-150. Prussin, C., & Metcalfe, D. D. (1995). Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. Journal of immunological methods, 188(1), 117-128. Andersen, H., Rossio, J. L., Coalter, V., Poore, B., Martin, M. P., Carrington, M., & Lifson, J. D. (2004). Characterization of rhesus macaque natural killer activity against a rhesus-derived target cell line at the single-cell level. Cellular immunology, 231(1), 85-95. |