Anti-Human CD45RA BG Violet 450
The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.
BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
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Clone
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HI100
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Format: |
BG Violet 450
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Applications
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FC
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Reactivity
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Human
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Isotype:
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Mouse IgG2b, kappa
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989. Barclay, A. N., Brown, M. H., Law, S. A. K. A., McKnight, A. J., Tomlinson, M. G., & van der Merwe, P. A. (1997). The leucocyte antigen factsbook. Academic Press. Yamada, T., Zhu, D., Saxon, A., & Zhang, K. (2002). CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase. Journal of Biological Chemistry,277(32), 28830-28835. |