Product Name :
Microcystin-LR monoclonal antibody (MC10E7)
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Storage at 4°C will decrease antibody activity and must be avoided.
Description:
With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized.{{2798856-04-7} web|{2798856-04-7} Biological Activity|{2798856-04-7} In Vitro|{2798856-04-7} supplier} The affinity constant for MC10E7 was determined to be at least 7×1010 l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable.{{2577204-16-9} MedChemExpress|{2577204-16-9} Protocol|{2577204-16-9} Description|{2577204-16-9} custom synthesis} Microcystin-LR spiked water samples in the concentration range between 0.PMID:31082092 01 and 0.1µg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
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| Application ELISA, IHC (PS), WB | Application Notes Microcystin concentrations can be measured in the range of ~0.006µg/l to 0.3µg/l. The ELISA sensitivity is about 1’000 times higher compared to UV detection. | Clone MC10E7 | Formulation Lyophilized. | Host Mouse | Immunogen Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin. | Isotype IgG1 | Recommendation Dilutions/Conditions ELISA (1:10,000-1:100,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Reconstitution Reconstitute with 200µl PBS containing sodium azide. | Species Reactivity Species independent | Specificity Recognizes all 4-Arg microcystins. | Unit of Measure (UM) µg