Product Name :
Human Mature TGF beta 1 Protein
EN
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1
Description:
| Human Mature TGF beta 1 Protein with No Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human Mature TGF beta 1 Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.
Source:
The recombinant Human Mature TGF beta 1 Protein, (amino acids Ala279-Ser390) fused with No Tag, was produced in HEK 293 cells.
Molecular Weight :
The recombinant Human Mature TGF beta 1 Protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity:
Immobilized recombinant Human Mature TGF beta 1, No tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human TGF-beta RII, mFc tag with the EC50 of 3.8 ng/ml determined by ELISA (QC Test). The affinity constant of 0.35 uM as determined in SPR assay (Biacore T200).
Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.
Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human Mature TGF beta 1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping:
The recombinant Human Mature TGF beta 1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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