Anti-Mouse IL-1 alpha SAFIRE Purified
IL-1α is a non-secreted, proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. The ALF-161 monoclonal antibody reacts with the precursor, secreted and membrane-associated forms of mouse IL-1 α. The antibody is reported to be usable as the capture antibody in an ELISA assay and exhibits neutralization properties. VX-765
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Clone
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ALF-161
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Format: |
SAFIRE Purified
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Applications
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FA, FC, Neutralization
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Reactivity
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Mouse
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Isotype:
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Armenian Hamster IgG
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Preperation:
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The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography.
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Formulation:
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Phosphate-buffered aqueous solution, ph7.2.
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References:
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Shibuya, K., Robinson, D., Zonin, F., Hartley, S. B., Macatonia, S. E., Somoza, C., … & O’Garra, A. (1998). IL-1α and TNF-α are required for IL-12-induced development of Th1 cells producing high levels of IFN-γ in BALB/c but not C57BL/6 mice. The Journal of Immunology, 160(4), 1708-1716. Fuhlbrigge, R. C., Sheehan, K. C., Schreiber, R. D., Chaplin, D. D., & Unanue, E. R. (1988). Monoclonal antibodies to murine IL-1 alpha. Production, characterization, and inhibition of membrane-associated IL-1 activity. The Journal of Immunology, 141(8), 2643-2650. Beckerman, K. P., Rogers, H. W., Corbett, J. A., Schreiber, R. D., McDaniel, M. L., & Unanue, E. R. (1993). Release of nitric oxide during the T cell-independent pathway of macrophage activation. Its role in resistance to Listeria monocytogenes. The Journal of Immunology, 150(3), 888-895. |