Anti-Human/Mouse CD11b SAFIRE Purified
The M1/70 monoclonal antibody specifically reacts with the 170 kDa αM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.
M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.
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Clone
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M1/70
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Format: |
SAFIRE Purified
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Applications
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FC, FA, IHC
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Reactivity
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Human, Mouse
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Isotype:
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Rat IgG2b, kappa
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| Cell Type: | , |
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Preperation:
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The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography.
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Formulation:
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Phosphate-buffered aqueous solution, ph7.2.
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References:
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Sanchez-Madrid, F., Simon, P., Thompson, S., & Springer, T. A. (1983). Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.The Journal of experimental medicine, 158(2), 586-602. Ault, K. A., & Springer, T. A. (1981). Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. The Journal of Immunology, 126(1), 359-364. Springer, T., Galfre, G., Secher, D. S., & Milstein, C. (1978). Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. European journal of immunology, 8(8), 539-551. |