Anti-Human CD28 SAFIRE Purified
The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.
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Clone
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CD28.2
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Format: |
SAFIRE Purified
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Applications
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FC, FA, IHC, IP
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Reactivity
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Human
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Isotype:
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Mouse IgG1, kappa
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Preperation:
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The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
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Formulation:
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Phosphate-buffered aqueous solution, ph7.2.
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References:
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Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York Nunès, J., Klasen, S., Ragueneau, M., Pavon, C., Couez, D., Mawas, C., … & Olive, D. (1993). CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events.International immunology, 5(3), 311-315. Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., & Vaslin, B. (2007). FoxP3+ CD25+ CD8+ T-cell induction during primary simian immunodeficiency virus infection in cynomolgus macaques correlates with low CD4+ T-cell activation and high viral load. Journal of virology, 81(24), 13444-13455. |