Product Name :
Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein

EN
HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1

Description:
| Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein with the C-His-Avi Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.

Source:
The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein, (amino acids Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.

Molecular Weight :
The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer protein with the His-Avi tag at the C-terminus has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Activity:

Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.

Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.

Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping:
The recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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