Way have been identified by KEGG enrichment annotation (Fig. four: 5). KEGG evaluation showed
Way have been identified by KEGG enrichment annotation (Fig. 4: 5). KEGG evaluation showed that compared with CAK (BR spraying for 0 h), the expression of the UTPglucose-1-phosphate uridylyltransferase (UGP), SPS, glucose-6-phosphate isomerase (GPI), pyrophosphateJin et al. BMC Genomics(2022) 23:Web page ten ofFig. 5 A feasible model on the BR signaling pathway with BRs (the activation state of BR signaling) sprayed onto tea leavesJin et al. BMC Genomics(2022) 23:Page 11 offructose-6-phosphate 1-phosphotransferase (PFP), and epidermis-specific secreted glycoprotein (EP) P2Y6 Receptor supplier important regulatory genes related towards the sucrose biosynthesis pathway have been upregulated following BR spraying for three h, 9 h, 24 h, and 48 h.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes in the biosynthetic pathway of flavonoidsEleven genes involved in flavonoid biosynthesis have been identified by KEGG enrichment annotation (Fig. 4: six). The flavonoid biosynthesis-related genes PAL, C4H, 4CL, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3,5-hydroxylase (F3’5’H), DFR, LAR, ANR, and UFGT were upregulated, with peak values observed at 48 h.DiscussionBR signal transduction mechanism in tea leavesThrough KEGG enrichment and annotation, 26 genes involved in the BR signal transduction pathway had been identified. Based on the heat maps of genes associated to BR signal transduction beneath various BR remedies, it was discovered that 26 genes within the BR signal transduction pathway have been drastically upregulated with growing BR spraying time. Combined using the BR signal transduction maps of Arabidopsis and rice, we Transthyretin (TTR) Inhibitor Formulation describe a possible model from the BR signal pathway in tea leaves [291] (Fig. five). At present, the signal transduction pathway of BR in Arabidopsis and rice has been reported. Compared with rice, the signal transduction pathway of BR in tea leaves is related to that of Arabidopsis [24]. In contrast to the BR signal transduction pathway in a. thaliana, BAK1-like kinase contains both SERK and TMK4 in the BR signal transduction pathway of tea leaves. In our transcriptome data, the ATBS1-interacting things (AIF) and paclobutrazol resistance 1 (PRE) genes didn’t significantly differ in expression levels, whereas that in the teosinte branched (TCP) gene was important. AIF is definitely the unfavorable regulator of BR signal transduction, while PRE and TCP are the constructive regulators of BR signal transduction [34]. The results showed that TCP, the forward regulator of BR signal transduction, plays a major function within the effects from the exogenous spraying of BRs onto young tea leaves.Exogenous spraying of BR promotes the development and development of tea plantsGBSS, and SBE genes related to starch synthesis; and the flavonoid biosynthesis-related PAL, C4H, 4CL, CHS, CHI, F3H, F3’5, DFR, LAR, ANR, and UFGT genes were identified. The results showed that exogenous spraying of BRs upregulated the expression of genes related to sucrose synthesis, chlorophyll synthesis, starch synthesis, and flavonoid biosynthesis. It can be inferred that exogenous BR spraying enhanced the content material of sucrose, chlorophyll, starch, and flavonoids. Furthermore, a sizable quantity of extremely expressed cyclin genes, which includes Cyc, CycD3, CycD4, and CDC6, had been located. Cell cycle regulatory proteins can bind to cell differentiation cycle-coding proteins and activate corresponding protein kinases, thus promoting cell division. BRs can enhance plant growth by advertising cell division.