IDH1 Enzyme

Description:
|Introduction Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. |Reco|E. coli-derived. A single, non-glycosylated polypeptide chain containing 434 amino acids (1-414) and having a molecular mass of 48.8 kDa. IDH1 is fused to a 20 amino acid His-tag at N-terminus. The Specific activity is > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C. Purity > 95%, by SDS-|The IDH1 solution (1 ug/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF and 20% glycerol. Shipping: The product is shipped at a|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. |Other IDH1 Enzymes: Recombinant Yeast Isocitrate Dehydrogenase-1 Enzyme