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Urinary prostacyclin ELISA kit
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Quantitate PGI2 – measuring the stable breakdown products 6-keto-PGF1α and 2,3-dinor-6keto-PGF1α in one simple assayLess stress – much easier than GC/MS with results from up to 39 samples in duplicate in under 3 hoursSpecific – low cross-reactivity with related eicosanoids The Urinary Prostacyclin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 2 by measuring the stable metabolites in one simple assay.Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α. 6-keto-PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2.2456407-94-4 web Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF1α.4091-99-0 References The conversion of PGI2 into 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α is shown below. Urinary Prostacyclin ELISA Kit Urinary Prostacyclin ELISA Kit
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| Alternative Name PGI2, Prostaglandin I2 | Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of urinary prostacyclin metabolites in culture supernatants and urine from any species.PMID:28722955 | Assay Time | Contents DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution | Sensitivity 6.58 pg/ml (range 7.81 – 2,000 pg/ml) | Species Reactivity Species independent | Wavelength 405 nm