Product Name :
SEEBRIGHT® Red 650 dUTP
Sequence:
Purity:
≥93% (HPLC)
Molecular Weight:
Solubility :
Appearance:
Blue liquid.
Use/Stability :
As indicated on product label or CoA when stored as recommended. Stable for at least one year after receipt when stored as recommended.
Description:
Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.2591300-72-8 Formula Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3′-end labeling.Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 650 dUTP can also be used for multicolor fluorescence labeling.This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 . Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis. Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.92915-79-2 supplier SEEBRIGHT™ Red 650 dUTP image Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.PMID:30422588 Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis. SEEBRIGHT™ Red 650 dUTP image
CAS :
Solubility:
Formula:
Additional Information :
| Alternative Name Enhanced Cyanine 5-dUTP | Appearance Blue liquid. | Concentration 1mM | Correction Factor (260nm) 0.05 | Correction Factor (280nm) 0.05 | Emission Maximum 662 nm | Excitation Maximum 650 nm | Extinction Coefficient 250,000 M-1 cm-1 (650 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) | Formulation Liquid. Solution in water. | Purity ≥93% (HPLC) | Purity Detail Purified by ion-exchange chromatography. | Quantity Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111. | Technical Info / Product Notes Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.