Product Name :
[pThr183/Tyr185]Jnk1/2 ELISA kit

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Store all components at 4°C, except standard at -20°C.

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Sensitive – use less precious sample per test compared to Western blot Higher throughput – test up to 40 samples in duplicate in just 3 hours Quantitative – obtain statistically signficant results and compare data between multiple experiments Ready-to-use – save time and reduce errors with pre-coated plates The [pThr183/Tyr185]Jnk1/2 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.The Stress-activated protein kinases alpha and γ (SAPKα and γ), also know as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinase belonging to the MAP kinase family. The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity. [pThr183/Tyr185]Jnk1/2 ELISA kit Kit graph [pThr183/Tyr185]Jnk1/2 ELISA kit Kit graph

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| Alternative Name c-Jun N-terminal kinase 1/2, SAP kinase 1a/c, MAP kinase 8/9, MAPK8/9 | Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of [pThr183/Tyr185]Jnk1/2 in cell lysates of human, mouse, and rat origin.{{2360851-29-0} medchemexpress|{2360851-29-0} Technical Information|{2360851-29-0} In Vitro|{2360851-29-0} manufacturer} | Assay Time 3 hours | Contents Microtiter plate, Conjugate, Antibody, Assay buffer 4 concentrate, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2 | Crossreactivity Phospho Jnk1 (100%), phospho Jnk2 (98.{{2563855-03-6} site|{2563855-03-6} Biological Activity|{2563855-03-6} Purity|{2563855-03-6} custom synthesis} 6%), phospho p38 (0.PMID:29999875 42%), phospho Erk2 (0.16%) and | Sensitivity 75.8 pg/ml (range 125 – 4,000 pg/ml) | Species Reactivity Human, Mouse, Rat | UniProt ID P45983 (Jnk1), P45984 (Jnk2) | Wavelength 450 nm

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