Product Name :
Phosphoserine monoclonal antibody (16B4)
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Stable at -80°C up to 1 year, at 4°C up to 1 week.
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| Application ELISA, IP, WB | Clone 16B4 | Formulation Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. | Host Mouse | Immunogen Phosphoserine containing peptides conjugated to KLH. | Isotype IgM | Positive Control Included. | Recommendation Dilutions/Conditions ELISA (use at 0.{{1570496-34-2} medchemexpress|{1570496-34-2} Technical Information|{1570496-34-2} In Vivo|{1570496-34-2} supplier} 05µg/ml)Western Blot (1µg/ml HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.{{328968-36-1} web|{328968-36-1} Technical Information|{328968-36-1} In stock|{328968-36-1} supplier} 1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!)Suggested dilutions/conditions may not be available for all applications.PMID:30725688 Optimal conditions must be determined individually for each application. | Reconstitution Reconstitute with 1ml water (15 minutes at room temperature). | Species Reactivity Species independent | Specificity Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, strongly interacting with pSK and pSP motifs, thus specifically recognizing substrates of MAP/SAP kinases and CDC kinases. Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence. | Technical Info / Product Notes Replacement for ADI-905-805 | Unit of Measure (UM) µg