Product Name :
Human TEM1 Protein

EN
Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479

Description:
| Human TEM1/CD248 Protein with the C-His Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human TEM1/CD248 Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.

Source:
The recombinant Human TEM1/CD248 Protein, (amino acids Gln18-Leu687) fused with a His tag at the C-terminus, was produced in HEK 293 cells.

Molecular Weight :
The recombinant Human TEM1/CD248 protein with the His tag at the C-terminus has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.

Activity:
Immobilized recombinant Human TEM1 protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-TEM1 Antibody protein with the C-hFc tag with the EC50 of 2.0 ng/ml determined by ELISA.

Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.

Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.

Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human TEM1/CD248 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping:
The recombinant Human TEM1/CD248 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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