Product Name :
Human Nectin-3 Protein

EN
CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3

Description:
| Human Nectin-3/CD113 Protein with the C-His-Avi Tag |Source: The reco|The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human Nectin-3/CD113 Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.

Source:
The recombinant Human Nectin-3/CD113 Protein, (amino acids Gly58-Asp400) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.

Molecular Weight :
The recombinant Human Nectin-3/CD113 protein with the His-Avi tag at the C-terminus has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Tris-Bis PAGE result.

Activity:
Immobilized recombinant Human Nectin-3 protein with the C-His-Avi tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human Nectin-1 protein with the C-hFc tag with the EC50 of 14.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).

Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.

Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.

Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human Nectin-3/CD113 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping:
The recombinant Human Nectin-3/CD113 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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