Product Name :
8-Azaadenosine
Description:
8-Azaadenosine is a potent ADAR1 inhibitor and an A-to-I editing inhibitor. 8-Azaadenosine blocks RNA editing and inhibits proliferation, 3D growth, invasion, and migration in thyroid cancer cells.
CAS:
10299-44-2
Molecular Weight:
268.23
Formula:
C9H12N6O4
Chemical Name:
(2R,3R,4S,5R)-2-{7-amino-3H-[1,2,3]triazolo[4,5-d]pyrimidin-3-yl}-5-(hydroxymethyl)oxolane-3,4-diol
Smiles :
NC1=NC=NC2=C1N=NN2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
InChiKey:
OAUKGFJQZRGECT-UUOKFMHZSA-N
InChi :
InChI=1S/C9H12N6O4/c10-7-4-8(12-2-11-7)15(14-13-4)9-6(18)5(17)3(1-16)19-9/h2-3,5-6,9,16-18H,1H2,(H2,10,11,12)/t3-,5-,6-,9-/m1/s1
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life:
≥12 months if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
8-Azaadenosine is a potent ADAR1 inhibitor and an A-to-I editing inhibitor. 8-Azaadenosine blocks RNA editing and inhibits proliferation, 3D growth, invasion, and migration in thyroid cancer cells.|Product information|CAS Number: 10299-44-2|Molecular Weight: 268.23|Formula: C9H12N6O4|Chemical Name: (2R,3R,4S,5R)-2-{7-amino-3H-[1,2,3]triazolo[4,5-d]pyrimidin-3-yl}-5-(hydroxymethyl)oxolane-3,4-diol|Smiles: NC1=NC=NC2=C1N=NN2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O|InChiKey: OAUKGFJQZRGECT-UUOKFMHZSA-N|InChi: InChI=1S/C9H12N6O4/c10-7-4-8(12-2-11-7)15(14-13-4)9-6(18)5(17)3(1-16)19-9/h2-3,5-6,9,16-18H,1H2,(H2,10,11,12)/t3-,5-,6-,9-/m1/s1|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : 250 mg/mL (932.04 mM; Need ultrasonic).|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥12 months if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|8-Azaadenosine targets ADAR (adenosine deaminases acting on double-stranded RNA) activity only and does not affect ADAR1 mRNA levels. 8-Azaadenosine (10-25 nM) restores let-7 miRNA biogenesis commensurate with a reduction in ADAR1 expression, RNA editing activity and LIN28B expression in JAK2/BCR-ABL1 transduced progenitors after two weeks in stromal co-culture.{{Rhodamine B} site|{Rhodamine B} {Fluorescent Dye}|{Rhodamine B} Technical Information|{Rhodamine B} References|{Rhodamine B} custom synthesis|{Rhodamine B} Cancer} 8-Azaadenosine (10, 100 nM) shows no effect on BCR-ABL and JAK2 signaling, as demonstrated by qRT-PCR analysis and p-CRKL and p-STAT5a Western blot analysis.{{Apixaban} web|{Apixaban} Factor Xa|{Apixaban} Protocol|{Apixaban} Formula|{Apixaban} manufacturer|{Apixaban} Epigenetics} 8-Azaadenosine (0.PMID:24318587 1, 0.5, 1, 2 µM; 5 days) decreases cell viability/proliferation in a dose-dependent manner in TPC1 and Cal62 cells. 8-Azaadenosine (1, 2 µM; 16 hours) impedes invasion and migration of TPC1 and Cal62 cells. 8-Azaadenosine (1, 2 µM) inhibits the editing activity in TPC1 and Cal62 cells. By contrast, ADAR1 mRNA levels remains stable in both cells lines.|Products are for research use only. Not for human use.|