Product Name :
Human ICOS Protein

EN
AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850

Description:
| Human ICOS/CD278 (C136S, C137S) Protein with the C-His Tag |Source: The reco|>95% by SEC-HPLC and SDS-|Less than 1 EU per ug of protein as determined by LAL method. |Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |Reconstitution: It is recommended to reconstitute the lyophilized reco|The recombinant Human ICOS/CD278 (C136S, C137S) Protein is shipped with ice |Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 week, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -70°C under sterile conditions after reconstitution.

Source:
The recombinant Human ICOS/CD278 (C136S, C137S) Protein, (amino acids Glu21-Phe141(C136S,C137S)) fused with a His tag at the C-terminus, was produced in HEK 293 cells.

Molecular Weight :
The recombinant Human ICOS/CD278 (C136S, C137S) protein with the His tag at the C-terminus has a predicted MW of 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity:
Immobilized recombinant Human ICOS (C136S, C137S) protein with the C-His tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human B7-H2 protein with the C-hFc tag with the EC50 of 16.8 ng/ml determined by ELISA (QC Test). The affinity constant of 40.2 nM as determined in SPR assay (Biacore T200).

Purity :
>95% by SEC-HPLC and SDS-PAGE under reducing conditions.

Endotoxin:
LevelLess than 1 EU per ug of protein as determined by LAL method.

Formulation :
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution:
It is recommended to reconstitute the lyophilized recombinant Human ICOS/CD278 (C136S, C137S) Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping:
The recombinant Human ICOS/CD278 (C136S, C137S) Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage :
/strong>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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