Name : PDGF-BB, Human (P. pastoris-expressed)

Product Concentration :

Background :
Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.

Biological Activity :
ED50 3.3 × 105 units/mg.

expression system :
P. pastoris

Tag :
PDGF-BB (Ser82-Thr190)Accession # P01127

Protein Accession No. :
P01127

Synonyms :
PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B

Molecular Weight :
~24.3 kDa, on SDS-PAGE under non-reducing conditions.

Purity :
> 97% as analyzed by SDS-PAGE> 97% as analyzed by HPLC

Product Concentration :
Lyophilized after extensive dialysis against 10 mM acetic acid.

Storage and Stability :
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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