Name : LR3-IGF-I (Receptor Grade), Human

Product Concentration :

Background :
IGF-1 is a well-characterized basic peptide secreted by the liver that circulates in the blood. It has growth-regulating, Insulin-like, mitogenic activities. IGF-1 is a growth factor that has a major, but not absolute, dependence on somatotropin. It is believed to be mainly active in adults in contrast to IGF-2, which is also a major fetal growth factor. Human Long R3 Insulin-like Growth Factor-1 (rhLR3IGF-1) contains an 83 amino acid analog of human IGF-I. Compared to the complete human IGF-I sequence, an addition of the rhLR3IGF-1 includes the substitution of an Arg for the Glu at position 3 (hence R3)and a13 amino acid extension peptide at the N-terminus. An enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.

Biological Activity :
ED50 1.0 × 105 units/mg.

expression system :
E. coli

Tag :
Expressed with additional N-terminal sequence (MFPAMPLSSLFVNGPRT). LR3-IGF-I (Leu53-Ala118)Accession # P05019

Protein Accession No. :
P05019

Synonyms :
LR3 Insulin-like Growth Factor-I; LR3-IGF-I

Molecular Weight :
~9.1 KDa, on SDS-PAGE under reducing conditions.

Purity :
> 95% as analyzed by SDS-PAGE

Product Concentration :
Lyophilized from a solution in 48% acetonitrile, 0.1% TFA.

Storage and Stability :
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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