Ncesa. Madrid. Spain., Madrid, SpainPT01.Biodistribution and proteomics analysis of plasma-derived EVs from Fasciola hepatica infections Alicia Galiano1; Joan Segui-Barber2; Miriam Diaz-Varela2; Susana GarciaSilva3; Maria Trelis4; H tor Peinado3; Fernando Cantalapiedra5; Dolores Bernal4; Hernando A. del Portillo6; Antonio MarcillaBackground: The complexity from the pathogenesis of IL-6 Inhibitor drug inflammatory bowel illness has led towards the quest of empirically drug therapies, combining immunosuppressant agents, biological therapy and modulators of your microbiota. Helminth parasites happen to be proposed as an option remedy of those diseases based on the hygiene hypothesis, but ethical and healthcare complications arise. The identification of extracellular vesiclesThursday, 03 Mayon these secreted products opens a new field of investigation, since they exert potent immunomodulating effects. Procedures: Adult parasites were cultured in vitro and secreted extracellular vesicles had been purified and applied for immunizing both wild-type C57BL/6 and RAG1-/- mice. Control and immunized mice groups have been treated with dextran sulphate sodium 7 days right after final immunization to market experimental colitis. The severity of colitis was assessed by illness activity index and histopathological scores. Mucosal cytokine expression was evaluated by ELISA. The activation of NF-kB, COX-2 and MAPK was evaluated by immunoblotting. Final results: Aurora A Inhibitor custom synthesis Injection of extracellular vesicles from F. hepatica (FhEVs) ameliorated the pathological symptoms induced by DSS in C57BL/6 mice measured by disease activity index, altering pro-inflammatory molecules in the intestine (TNF-, IL-6 and IL-17A), and interfering with both MAPK and NF-kB pathways. RAG1-/- mice treated with FhEVs showed preservation of tissue architecture whereas colitic mice displayed significant disruption areas from the colonic architecture. Summary/conclusion: Our final results indicate that extracellular vesicles from parasitic helminths can modulate immune responses in DSSinduced colitis, exerting a protective impact that must be mediated by other cells distinct from B- and T-lymphocytes. Funding: Supported by the Conselleria d’Educaci Cultura i Esports, Generalitat Valenciana, Valencia, Spain (PROMETEO/2016/156 to A. M.), Fundaci Ram Areces and REDIEX-Spanish Ministry of Economy and Competitiveness (MINECO) to A.M. and F.S.-M. F.SM was supported by MINECO (SAF2014-55579-R), Comunidad de Madrid, Spain (INDISNET-S2011/BMD-2332), as well as the European Investigation Council (ERC-2011-AdG 294340-GENTRIS). JR is supported by a Generalitat Valenciana (Valencia, Spain) predoctoral fellowship. MLS is supported by FPI programme (Spanish Ministry of Economy).implying a role in parasite-driven immunomodulation. Furthermore, we demonstrated that T. muris EVs is often actively internalized by mouse colonic organoids, suggesting a function in host arasite communication. Summary/conclusion: Understanding how parasites interact with their hosts is vital to create new manage measures. This very first characterization with the proteins and nucleic acids in the EVs secreted by T. muris offers critical details on whipworm ost communication and forms the basis for future research. Funding: This work was supported by a program grant in the National Well being and Healthcare Study Council (NHMRC) [program grant number 1037304] in addition to a Principal Study fellowship from NHMRC to AL. RME was supported by an Early Postdoc Mobility Fellowship (P2ZHP3_161693) in the Swiss National Scien.