Y/Conclusion: Mare endometrial cells display principal biological properties of adipose MSCs, the MV/EXo secretion KIR2DS1 Proteins Recombinant Proteins pattern was also related in terms of typical particle size and kinetics. At 75 confluence, there is a larger quantity of Exo secreted by cells from each origins. The traits of this exosomes remain to be tested and deep sequencing is becoming carried at present Funding: This function was funded by Grant Fondecyt [1150757], Government of ChilePF03.12 = OWP.1.Osteoblast-secreted extracellular vesicles stimulate the expansion of CD34+ human umbilical cord blood cellsPF03.Mesenchymal stromal cell Complement Factor P Proteins Molecular Weight derived extracellular vesicles show distinct chondrogenesis microRNA expression profiles from their parental cells Rachel E. Crossland1; Monica Reis2; Matthew J. Barter3; Lindsay Nicholson1; David A. Young3; Anne M. Dickinson1; Xiao-nong Wang1 Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK; Division of Pediatrics, Harvard Health-related School, Boston, MA, USA; 3Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, UK2PF03.Pattern of secretion in vitro of microvesicles and exosomes in equine mesenchymal stem cells derived from the exact same animals but from diverse tissues Navarrete Felipe1; Cabezas Joel1; Daniela Rojas2; Andrea Navarro1; Pedro Pablo Silva2; JosManr uez1; Fernando Saravia2; Lleretny Rodr uezAlvarez1; Fidel Ovidio Castro1 Division of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepcion, Chillan, Chile; 2Department of Pathology, Faculty of Veterinary Sciences, Universidad de Concepcion, Chill , Chile; 3Universidad de Concepci , Chill , ChileBackground: Mesenchymal stem cells (MSC) have already been postulated as responsible for cell and tissue renewal, they play an essential immunomodulatory role and exert their action mainly through paracrine signaling. Microvesicles (MVs) and exosomes (Exo) had been identified as essential player in regulation of biological action. Based on their niche, the regenerative and immunomodulatory properties of MSC may vary as well as their MVs/Exo pattern. Here we compared the MV/Exo pattern of MSC derived from different tissues of your similar animals Methods: Six major cell cultures have been derived and expanded in the adipose and endometrial tissue of three different mares. Population doubling time (PDT), colony formation (CF) and surface expression of MSC markers (FACS) were studied. At P2, cells have been subjected to differentiation and staining at 0,7,14 and 21 days. Migration experiments making use of “scratch method” have been performed. Each and every experiment was replicated 3X, controls had been included. For MV/Exo evaluation, MSC had been cultured in DMEM+10 FCS. At 50 , 75 and 90 of confluence, medium was changed and cells were further cultured for 48 h in exact same medium but depleted of MV/Exo by serial ultracentrifugation. The supernatant was collected and subjected to NTA (Nanosight NS300). Outcomes were analysed according to tissue of origin and confluence applying various comparisonBackground: Mesenchymal stromal cells (MSCs) are often utilised in clinical trials for wide-ranging immunological and degenerative ailments. MSC-secreted extracellular vesicles (MSC-EVs) are increasingly reported as the crucial paracrine aspects accountable for MSC clinical benefit, indicating their possible as a cell-free therapy for regenerative medicine. However, the role of MSC-EVs in MSC biology is largely unknown and their molecular composition has not been completely characterized. Here we re.