Uret et al. This pceABCT operon is conserved in the exact same genome context in all 5 Dehalobacter genomes,while considerable sequence variations within this operon exists among these five Dehalobacter strains,indicating that the pceABCT operon has been carried by each Dehalobacter for some time (Figure S). Interestingly,in the genomes of strain CF and strain DCA,this operon is situated within a area flanked by two direct repeats ( bp each) with other repeat patterns,indicating that the operon could have been acquired horizontally. These repeat patterns were not found in strain PERK nor in strain E. No other evidence for the presence of genomic islands or horizontal gene transfer events involving rdhA genes was located in any of your five Dehalobacter genomes. Horizontal gene transfer appears to play a vital part in shaping Dehalobacter genomes. As indicated previously,significant sequence variations between the five genomes are positioned in regions A,B,and C highlighted in Figure . Region A features a current insertion of a kb fragment (A) within the genome of strain CF (Figure. This region includes a fairly low GC contentFrontiers in Microbiology www.frontiersin.orgFebruary Volume ArticleTang et alparative Dehalobacter Genome AnalysisFIGURE Three potential genome rearrangements in between Dehalobacter restrictus strain PERK and Dehalobacter sp. strain CF. Note the blocks with identical color represent corresponding areas between the genomes of strain CF and strain PERK with higher similarity. Panel (A) shows the Mauve alignment and original GC skew profile of your two genomes. Panels (B represent the step artificial reversion scenario that could clarify the genome rearrangements observed involving the two strains. Actions and in Panels (B,C) describe two sequence inversions,and step in Panel (D) represents a translocation. The resulting alterations in Mauve alignments are depicted with corresponding modifications in GC skew profiles shown for the right.( and most genes involved are special to strain CF. The insertion is likely related to a phage integrase (DCF_p) positioned at the end of A (Figure. In Area C,which features a low GC content material (strain CF has quite a few phagerelated genes. Incorporation of area C (Figure into strain CF is probably related to a sitespecific recombinase (DCF_p)located in the finish of C. Alternatively,sequence C from strain DCA is kb long and includes a GC content material of . ,related towards the typical GC content material of the complete genome,and could indicate that C is native to Dehalobacter. In region B,both B (strain CF) and B (Strain DCA) have a low GC content,and poor sequence conservation was found between allFrontiers in Microbiology www.frontiersin.orgFebruary Volume ArticleTang et alparative Dehalobacter Genome Analysisfive Dehalobacter genomes,indicating a hypervariable region. Area D (Figure is an additional large phage elated region MedChemExpress Cyclic somatostatin shared by both strain CF and DCA,but not discovered in strain PERK and E. Region D is kb extended and contains lots of genes encoding either phagerelated or hypothetical proteins,which includes a phage integrate situated at the finish on the region. This insertion occasion targeted tRNAThr (DCF_r) and resulted in duplication ( bp) in the insertion web site,which consists of a partial tRNAThr gene.Metabolic PotentialWe examined in detail the gene annotations of Dehalobacter to reveal metabolic potential and any variations among the strains. All round,these Dehalobacter strains are highly comparable. Genes for PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20972551 all major metabolic pathways and physiological specifications of.