Propidium Iodide Solution
The PI (Propidium iodide) solution can be used to exclude non-viable cells in flow cytometry assays. PI is a fluorescent dye that binds to double stranded DNA in cells that have lost plasma membrane integrity, such as those in the late stages of apoptosis or dead cells. It is detected in the orange 562-588 nm band-pass filter. Linsitinib
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Applications
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FC
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Application Notes:
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Formulation:
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References:
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Martin, S., Reutelingsperger, C. P., McGahon, A. J., Rader, J. A., Van Schie, R. C., LaFace, D. M., & Green, D. R. (1995). Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. The Journal of experimental medicine, 182(5), 1545-1556. Douglas, R. S., Tarshis, A. D., Pletcher Jr, C. H., Nowell, P. C., & Moore, J. S. (1995). A simplified method for the coordinate examination of apoptosis and surface phenotype of murine lymphocytes. Journal of immunological methods,188(2), 219-228. |