Anti-Mouse CD62L (L-Selectin) APC-Cyanine7
The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin: 74 kDa (on lymphocytes) or 95 kDa (on neutrophils).In the Mouse organism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34). It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules). In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes. LY2811378
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Clone
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MEL-14
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Format: |
APC-Cyanine7
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Applications
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FC
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Reactivity
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Human
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Isotype:
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Rat IgG2a, kappa
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Lanza, F., Bi, S., Castoldi, G., & Goldman, J. M. (1993). Abnormal expression of N-CAM (CD56) adhesion molecule on myeloid and progenitor cells from chronic myeloid leukemia. Leukemia, 7(10), 1570-1575. Reimann, K. A., Waite, B. C., Lee‐Parritz, D. E., Lin, W., Uchanska‐Ziegler, B., OConnell, M. J., & Letvin, N. L. (1994). Use of human leukocyte‐specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry, 17(1), 102-108. Carter, D. L., Shieh, T. M., Blosser, R. L., Chadwick, K. R., Margolick, J. B., Hildreth, J. E. K., … & Zink, M. C. (1999). CD56 identifies monocytes and not natural killer cells in rhesus macaques. Cytometry, 37(1), 41-50. |