Anti-Mouse CD273 (PD-L2, B7-DC) Purified
The TY25 antibody reacts with mouse B7-DC (PD-L2), a 42 kDa glycoprotein of the B7 family of the immunoglobulin superfamily. B7-DC is expressed by macrophages, monocytes, and sub-populations of dendritic cells. B7-DC has similarities with the rest of the B7 family, but it does not bind to CD28/CD152. The receptor for B7-DC is PD-1, which contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), and is expressed on activated B and T cells. The interaction between B7-DC and its receptor seems to downregulate immune responses. However, B7-DC seems to be a costimulator of T lymphocytes, reacting with a receptor different than PD-1. B7-DC can bind and stimulate dendritic cells. It is also the first B-7 ligand involved in bidirectional communication.
The TY25 monoclonal antibody blocks the binding of B7-DC to its receptor (PD-1) and can be used for studying the interaction between the antigen presenting cells and T lymphocytes.
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Clone
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TY25
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Format: |
Purified
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Applications
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FC, FA, IHC, IF, IP, WB
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Reactivity
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Mouse
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Isotype:
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Rat IgG2a, kappa
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Tseng, S. Y., Otsuji, M., Gorski, K., Huang, X., Slansky, J. E., Pai, S. I., … & Tsuchiya, H. (2001). B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. The Journal of experimental medicine,193(7), 839-846. Wang, S., Bajorath, J., Flies, D. B., Dong, H., Honjo, T., & Chen, L. (2003). Molecular modeling and functional mapping of B7-H1 and B7-DC uncouple costimulatory function from PD-1 interaction. The Journal of experimental medicine, 197(9), 1083-1091. Aramaki, O., Shirasugi, N., Takayama, T., Shimazu, M., Kitajima, M., Ikeda, Y., … & Niimi, M. (2004). Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation, 77(1), 6-12. |