Anti-Human CD80 (B7-1) SAFIRE Purified
The 2D10.4 antibody reacts with human CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Y-27634 (dihydrochloride)
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Clone
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2D10.4
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Format: |
SAFIRE Purified
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Applications
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FC, FA
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Reactivity
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Human
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Isotype:
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Mouse IgG1, kappa
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Preperation:
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The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
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Formulation:
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Phosphate-buffered aqueous solution, ph7.2.
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References:
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Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998. Cognasse, F., Hamzeh‐Cognasse, H., Lafarge, S., Chavarin, P., Pozzetto, B., Richard, Y., & Garraud, O. (2008). Identification of two subpopulations of purified human blood B cells, CD27− CD23+ and CD27high CD80+, that strongly express cell surface Toll‐like receptor 9 and secrete high levels of interleukin‐6. Immunology, 125(3), 430-437. Bashuda, H., Kimikawa, M., Seino, K., Kato, Y., Ono, F., Shimizu, A., … & Okumura, K. (2005). Renal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates. Journal of Clinical Investigation, 115(7), 1896-1902. |