Anti-Human CD54 (ICAM-1) FITC
The 15.2 antibody reacts with the 85-110 kDa intracellular adhesion molecule-1 (ICAM-1), a member of the Ig superfamily which acts as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1). ICAM-1 is also known CD 54 and is expressed on non-hematopoietic cells of vascular endothelial, thymic epithelial, fibroblasts lineages, and on hematopoietic cells like macrophages, mitogen-stimulated T-lymphoblasts, dendritic cells in tonsils, lymph nodes and Peyer’s patches, and germinal center B cells.
Inflammatory mediators (IL-1, TNF, IFN-γ) enhance the production of ICAM-1 on fibroblasts and endothelial cells within few hours. Thus, ICAM-1 seems to be the marker of inflammatory reactions.
|
Clone
|
15.2
|
|
Format: |
FITC
|
|
Applications
|
FC
|
|
Reactivity
|
Human
|
|
Isotype:
|
Mouse IgG1
|
| Research Interest: | , |
| Cell Type: | , , , |
| Protein Cross Reactivity: | Pig |
|
Preperation:
|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
|
|
Formulation:
|
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
|
|
References:
|
Bhattacharya, A. L. O. K., Dorf, M. E., & Springer, T. A. (1981). A shared alloantigenic determinant on Ia antigens encoded by the IA and IE subregions: evidence for I region gene duplication. The Journal of Immunology, 127(6), 2488-2495. Mendiratta, S. K., Singh, N., Bal, V., & Rath, S. (1996). Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity.Immunology, 89(2), 238-244. Unternaehrer, J. J., Chow, A., Pypaert, M., Inaba, K., & Mellman, I. (2007). The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proceedings of the National Academy of Sciences,104(1), 234-239. |