Anti-Human CD49d FITC
The 9F10 monoclonal antibody specifically reacts with human CD49d, the 150 kDA alpha 4 integrin chain. The molecule forms the heterodimer called VLA-4 with integrin beta 1 and another heterodimer with integrin beta 7 that binds fibronectin, VCAM-1, and MadCAM-1. CD49d is expressed on monocytes, lymphocytes, thymocytes, NK cells, B cells, and T cells. It is involved in hematopoietic stem cell differentiation, cell migration, and cell activation. Its absence on Foxp3+ cells make it a useful marker to isolate Treg cell populations. MK-1777
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Clone
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9F10
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Format: |
FITC
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Applications
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FC
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Reactivity
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Human
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Isotype:
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Mouse IgG1, kappa
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Schlossman, S. F. (1995). Leucocyte typing V: White cell differentiation antigens: Proceedings of the Fifth International Workshop and Conference, Held in Boston, USA 3-7 November, 1993. Oxford University Press. Kleinewietfeld, M., Starke, M., Di Mitri, D., Borsellino, G., Battistini, L., Rötzschke, O., & Falk, K. (2009). CD49d provides access to “untouched” human Foxp3+ Treg free of contaminating effector cells. Blood, 113(4), 827-836. Hemler, M. E. (1990). VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annual review of immunology, 8(1), 365-400. |