Anti-Human CD45RO Purified
The UCHL1 monoclonal antibody specifically reacts with human CD45RO, a 180 kDa isoform of the leukocyte common antigen CD45. CD45RO is a transmembrane glycoprotein with tyrosine phosphatase activity and is expressed by majority of thymocytes, monocytes, granulocytes, and activated memory T lymphocytes. The subsets of peripheral T lymphocytes can be discriminated by using the CD45RO and CD45RA expressing cells.
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Clone
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UCHL1
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Format: |
Purified
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Applications
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FC, IHC, IF, IP, WB
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Reactivity
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Human
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Isotype:
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Mouse IgG2a, kappa
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| Research Interest: | |
| Cell Type: | , , |
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Preperation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989. Akbar, A. N., Terry, L., Timms, A., Beverley, P. C., & Janossy, G. (1988). Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. The Journal of Immunology, 140(7), 2171-2178. Smith, S. H., Brown, M. H., Rowe, D., Callard, R. E., & Beverley, P. C. (1986). Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology, 58(1), 63. |