Anti-Human CD32 SAFIRE Purified
The 6C4 monoclonal antibody specifically recognizes two isoforms of human CD32, Fc gamma RIIA and Fc gamma RIIB. From alternative mRNA splicing, 6 isoforms of CD32 have been reported. CD32 is a 40 kDA polymorphic transmembrane glycoprotein expressed on monocytes, macrophages, graulocytes, B cells, and platelets. The receptor has low affinity toward IgG and is involved in immunomodulation, phagocytosis, and the inflammation response. The 6C4 antibody in a rosette blocking assay is reported to inhibit Ig binding PA-824
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Clone
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6C4
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Format: |
SAFIRE Purified
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Applications
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FC, FA
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Reactivity
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Human
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Isotype:
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Mouse IgG1, kappa
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| Research Interest: | Inflammation |
| Cell Type: | , , |
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Preperation:
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The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
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Formulation:
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Phosphate-buffered aqueous solution, ph7.2.
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References:
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VELY, F., GRUEL, N., MONCUIT, J., COCHET, O., ROUARD, H., DARE, S., … & TEILLAUD, J. L. (1997). A new set of monoclonal antibodies against human FcγRII (CD32) and FcγRIII (CD16): characterization and use in various assays. Hybridoma, 16(6), 519-528. Liebert, M. A. (1997). 6C4, 2B2, 3D3. Hybridoma, 16(6). Bouhlal, H., Martinvalet, D., Teillaud, J. L., Fridman, C., Kazatchkine, M. D., Bayry, J., … & Kaveri, S. V. (2014). Natural Autoantibodies to Fcγ Receptors in Intravenous Immunoglobulins. Journal of clinical immunology, 1-8. |